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Genomic regions associated with the sex-linked inhibitor of dermal melanin in Silkie chicken

Ming TIAN,Rui HAO,Suyun FANG,Yanqiang WANG,Xiaorong GU,Chungang FENG,Xiaoxiang HU,Ning LI

《农业科学与工程前沿（英文）》 2014年 第1卷 第3期   页码 242-249 doi: 10.15302/J-FASE-2014018

摘要： A unique characteristic of the Silkie chicken is its fibromelanosis phenotype. The dermal layer of its skin, its connective tissue and shank dermis are hyperpigmented. This dermal hyperpigmentation phenotype is controlled by the sex-linked inhibitor of dermal melanin gene ( ) and the dominant fibromelanosis allele. This study attempted to confirm the genomic region associated with . By genotyping, was found to be closely linked to the region between GGA_rs16127903 and GGA_rs14685542 (8406919 bp) on chromosome Z, which contains ten functional genes. The expression of these genes was characterized in the embryo and 4 days after hatching and it was concluded that , encoding methylthioadenosinephosphorylase, would be the most likely candidate gene. Finally, target DNA capture and sequence analysis was performed, but no specific SNP(s) was found in the targeted region of the Silkie genome. Further work is necessary to identify the causal mutation located on chromosome Z.

关键词： sex-linked inhibitor of dermal melanin (Id)     Silkie     chromosome Z    

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Progress in studies of sex determination mechanisms and sex control techniques in

Qian ZHOU,Songlin CHEN

《农业科学与工程前沿（英文）》 2016年 第3卷 第2期   页码 113-123 doi: 10.15302/J-FASE-2016093

摘要： The (half-smooth tongue sole) is a marine flatfish of great commercial value for fisheries and aquaculture in China. It has a female heterogametic sex determination system (ZW/ZZ) and environmental factors can induce sex-reversal of females to phenotypic males, suggesting that it is a promising model for the study of sex determination mechanisms. Additionally, females grow much faster than males and it is feasible to improve the aquaculture production through sex control techniques. This paper reviews the progress in research on sex determination mechanisms research in our laboratory. We have completed whole-genome sequencing and revealed the genome organization and sex chromosome evolution of . A putative male determining gene was identified and DNA methylation was verified as having a crucial role in the sex reversal process. Genetic maps and sex-specific biomarkers have been used in a marker-assisted selection breeding program and for differentiation of the fish sex. Development and improvement of sex control technologies, including artificial gynogenesis and production of breeding fry with high proportion of females, is also reviewed. These research advances have provided insight into the regulation of sex determination and enabled efficient sex management in artificial culturing of .

关键词： Cynoglossus semilaevis     sex determination     sex control technique    

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Room temperature preparation of hydrazine-linked covalent organic frameworks coated capillaries for

《化学科学与工程前沿（英文）》 2023年 第17卷 第5期   页码 548-556 doi: 10.1007/s11705-022-2252-1

摘要： Covalent organic frameworks (COFs) have been increasingly used in capillary electrochromatography due to their excellent characteristics. In this work, hydrazine-linked TFPB-DHzDS (TFPB: 1,3,5-tris(4-formylphenyl)benzene; DHzDS: 2,5-bis(3-(ethylthio)propoxy)terephthalohydrazide) was first synthesized by a simpler and easier method at room temperature and introduced into capillary electrochromatography as coating material. The TFPB-DHzDS coated capillaries were prepared by an in-situ growth process at room temperature. After optimizing the coating concentration and experimental conditions of capillary electrochromatography, baseline separation of two groups of polycyclic aromatic hydrocarbons was achieved based on the TFPB-DHzDS coated capillary. And the established method was used successfully to determine PAHs in natural water and soil samples. The spiked recoveries of polycyclic aromatic hydrocarbons in these samples ranged from 90.01% to 111.0%, indicating that the method is reliable and could detect polycyclic aromatic hydrocarbons in natural samples. Finally, molecular simulation was applied to study and visualize the interaction between the analytes and coating COF materials to investigate the molecular level separation mechanism further.

关键词： hydrazine-linked TFPB-DHzDS     in-situ growth method     open-tubular capillary electrochromatography     molecular simulations    

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Kinematical synthesis of an inversion of the double linked fourbar for morphing wing applications

AVILéS, I. FERNáNDEZ, M. ABASOLO

《机械工程前沿（英文）》 2013年 第8卷 第1期   页码 17-32 doi: 10.1007/s11465-013-0364-5

摘要：

This paper presents the kinematical features of an inversion of the double linked fourbar for morphing wing purposes. The structure of the mechanism is obtained using structural synthesis concepts, from an initial conceptual schematic. Then, kinematic characteristics as instant center of rotation, lock positions, dead point positions and uncertainty positions are derived for this mechanism in order to face the last step, the dimensional synthesis; in this sense, two kinds of dimensional synthesis are arranged to guide the wing along two positions, and to fulfill with the second one some aerodynamic and minimum actuation energy related issues.

关键词： morphing wing     structural synthesis     dimensional synthesis     geometrical kinematics    

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The secondary laticifer differentiation in rubber tree is induced by trichostatin A, an inhibitor of

Shixin ZHANG,Shaohua WU,Weimin TIAN

《农业科学与工程前沿（英文）》 2016年 第3卷 第4期   页码 357-362 doi: 10.15302/J-FASE-2016125

摘要： The secondary laticifer, a specific tissue in the secondary phloem of rubber tree, is differentiated from the vascular cambia. The number of the secondary laticifer in the trunk bark of rubber tree is positively correlated with rubber yield. Although jasmonates have been demonstrated to be crucial in the regulation of secondary laticifer differentiation, the mechanism for the jasmonate-induced secondary laticifer differentiation remains to be elucidated. By using an experimental morphological technique, the present study revealed that trichostatin A (TSA), an inhibitor of histone deacetylation, could induce the secondary laticifer differentiation in a concentration-dependent manner. The results suggest that histone acetylation is essential for the secondary laticifer differentiation in rubber tree.

关键词： Hevea brasiliensis     histone acetylation     laticifer differentiation     trichostatin     vascular cambia    

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Dual cross-linked MXene/cellulose nanofiber/nickel alginate film with improved mechanical properties

《化学科学与工程前沿（英文）》 2023年 第17卷 第10期   页码 1460-1469 doi: 10.1007/s11705-023-2335-7

摘要： Electromagnetic interference pollution has raised urgent demand for the development of electromagnetic interference shielding materials. Transition metal carbides (MXenes) with excellent conductivity have shown great potential in electromagnetic interference (EMI) shielding materials, while the poor mechanical strength, flexibility, and structural stability greatly limit their further applications. Here, cellulose nanofibers and sodium alginate are incorporated with MXene nanosheets as flexible matrices to construct strong and flexible mussel-like layered MXene/Cellulose nanofiber/Sodium Alginate composite films, and nickel ions are further introduced to induce metal coordination crosslinking of alginate units. Benefited from the dual-crosslinked network structure of hydrogen bonding and metal coordination, the tensile strength, Young’s modulus, and toughness of the MXene/cellulose nanofiber/nickel alginate composite film are significantly increased. After subsequent reduction by ascorbic acid, excess nickel ions are reduced to nickel nanoparticles and uniformly dispersed within the highly conductive composite film, which further improved its hysteresis loss effect toward the incident electromagnetic waves. Consequently, the MXene/cellulose nanofiber/nickel alginate-Ni composite film presents a considerably enhanced electromagnetic interference shielding effectiveness (47.17 dB) at a very low thickness of 29 μm. This study proposes a feasible dual-crosslinking and subsequent reduction strategy to synergistically enhance the mechanical properties and electromagnetic interference shielding performance of MXene-based composite materials.

关键词： Ti3C2Tx MXene     double crosslinking     mechanical properties     EMI shielding performance    

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Identification of molecular markers linked to rice bacterial blight resistance genes from

Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN

《农业科学与工程前沿（英文）》 2015年 第2卷 第3期   页码 260-265 doi: 10.15302/J-FASE-2015070

摘要： Y73 is a progeny of asymmetric somatic hybridization between cv. Dalixiang and the wild rice species . Inoculation with a range of strains of pv. showed that Y73 had inherited a high level of resistance to rice bacterial blight (BB) from its wild parent. An F population of 7125 individuals was constructed from the cross between Y73 and a BB-susceptible cultivar IR24. After testing 615 SSR and STS markers covering the 12 rice chromosomes, 186 markers were selected that showed polymorphism between Y73 and IR24. Molecular markers linked to the BB resistance genes in Y73 were scanned using the F population and the polymorphic markers. The SSR marker RM128 on chromosome 1, the STS marker R03D159 on chromosome 3 and the STS marker R05D104 on chromosome 5 were found to be linked to the rice BB resistance genes in Y73.

关键词： Oryza meyeriana     bacterial blight resistance gene     genetic population     linkage molecular marker    

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利用<i>cyp17a1i>缺失的伪雄鲤成功构建全雌鲤群体 Article

翟刚, 舒婷婷, 陈贶新, 娄气永, 贾景怡, 黄建飞, 石闯 , 金霞, 贺江燕, 姜冬火, 钱雪桥, 胡炜, 殷战

《工程（英文）》 2022年 第8卷 第1期   页码 181-189 doi: 10.1016/j.eng.2021.03.026

摘要：

部分养殖鱼类存在两性生长异形的表型，因此，构建相应单性别养殖群体对水产养殖产量的提升具有产业应用价值。迄今为止，采用激素活性物质诱导产生性反转亲本是水产养殖业构建单性别群体最普遍使用的方法。本研究针对雌鲤生长快于雄鲤的两性生长异形的特性，以cyp17a1 为靶点进行了CRISPR/Cas9介导的基因编辑操作，利用cyp17a1 敲除后形成的伪雄鲤，成功构建了全雌鲤群体。首先，我们发现无论遗传性别型为XX还是XY，其cyp17a1 缺失鲤个体性腺均发育为精巢，并能正常产精。其次，利用确定的雄性特异DNA分子标记，筛选到cyp17a1 -/- XX伪雄个体，其具有正常的精巢发育和精子发生。将其与野生型雌鲤杂交，在8 月龄时检测后代性别，发现100%为雌性。将全雌群体和对照组雌雄混合群体各500 尾进行同塘生长对比养殖，至8 月龄或12 月龄时随机抽样检测，发现cyp17a1杂合突变雌鲤体重比同塘性别混养对照组分别高6.60%或32.66%。因此，本研究首次成功地利用基因工程技术对单基因位点进行操作，创制了全雌群体，实现了对雌鲤生长快的两性生长异形特性的应用，提升了养殖产量。

关键词： 鲤     两性异形     生长     <i>cyp17a1i>     性类固醇激素     全雌群体    

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Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein as a serum biomarker and implicates potential therapeutic targets

《医学前沿（英文）》 2022年 第16卷 第3期   页码 378-388 doi: 10.1007/s11684-021-0840-y

摘要： Macrolide and corticosteroid resistance has been reported in patients with Mycoplasma pneumoniae (MP) pneumonia (MPP). MP clearance is difficult to achieve through antibiotic treatment in sensitive patients with severe MPP (SMPP). SMPP in children might progress to airway remodeling and even bronchiolitis/bronchitis obliterans. Therefore, identifying serum biomarkers that indicate MPP progression and exploring new targeted drugs for SMPP treatment require urgency. In this study, serum samples were collected from patients with general MPP (GMPP) and SMPP to conduct proteomics profiling. The Fc fragment of the IgG-binding protein (FCGBP) was identified as the most promising indicator of SMPP. Biological enrichment analysis indicated uncontrolled inflammation in SMPP. ELISA results proved that the FCGBP level in patients with SMPP was substantially higher than that in patients with GMPP. Furthermore, the FCGBP levels showed a decreasing trend in patients with GMPP but the opposite trend in patients with SMPP during disease progression. Connectivity map analyses identified 25 possible targeted drugs for SMPP treatment. Among them, a mechanistic target of rapamycin kinase (mTOR) inhibitor, which is a macrolide compound and a cell proliferation inhibitor, was the most promising candidate for targeting SMPP. To our knowledge, this study was the first proteomics-based characterization of patients with SMPP and GMPP.

关键词： severe Mycoplasma pneumoniae pneumonia     children     proteomics     Fc fragment of the IgG-binding protein     mechanistic target of rapamycin kinase inhibitor    

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Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

《结构与土木工程前沿（英文）》 2014年 第8卷 第4期   页码 448-455 doi: 10.1007/s11709-014-0262-x

摘要： Rock mass classification system is very suitable for various engineering design and stability analysis. classification method is confirmed by Japan Highway Public Corporation that this method can figure out either strength or deformability of rock mass, further appropriating the amount of rock bolts, thickness of shotcrete, and size of pitch of steel ribs just after the blasting procedure. Based on these advantages of method, in this study, according to data of five deep and long tunnels in Iran, two equations for estimating the value of method from and classification systems were developed. These equations as a new method were able to optimize the support system for and classification systems. From classification and its application in these case studies, it is pointed out that the method for the design of support systems in underground working is more reliable than the and classification systems.

关键词： JH classification     Q and RMR classification     new method    

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抗白粉病基因 <i>Pm40i> 在我国“后 <i>Pm21i> 时代”小麦育种中的重要作用 Perspective

唐圣雯,胡玉亭,钟胜福,罗培高

《工程（英文）》 2018年 第4卷 第4期   页码 500-506 doi: 10.1016/j.eng.2018.06.004

摘要：

由小麦白粉菌[Blumeria graminis f. sp. tritici（Bgt）] 引起的小麦白粉病是一种重要的小麦叶部病害，对小麦的产量产生很大的影响。20 世纪80 年代，通过簇毛麦（Heuchera villosa）的6VS 染色体与小麦6AL 染色体易位将抗白粉病基因Pm21 转移到普通小麦中。最近，在一些地方发现了对Pm21 有毒的Bgt，虽然这些菌株的病理学特性还有待研究，但这一现象提醒小麦育种者应注意应用Pm21 的风险。来源于普通小麦与中间偃麦草（Thinopyrum intermedium）杂种后代的抗白粉病基因Pm40，被定位在小麦7BS 染色体上，对Bgt 具有广谱和持久的抗性。通过细胞学研究，并未在Pm40 的载体品种中发现大片段外缘染色体。过去几年的研究发现，Pm40 的载体品种具有优良的农艺性状。因此，我们相信在未来的育种工程中，Pm40 将会在Pm21 的抗性被克服之后起巨大的作用。另外，Pm21 和Pm40 都来源于外缘物种，这暗示着外源基因的抗性可能比小麦本身的基因更为持久和有效。

关键词： 小麦     白粉病     <i>Pm21i>     <i>Pm40i>     外源基因     内源抗性    

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NiFeRuO<i>xi> nanosheets on Ni foam as an electrocatalyst for efficient

《化学科学与工程前沿（英文）》 2023年 第17卷 第11期   页码 1698-1706 doi: 10.1007/s11705-023-2334-8

摘要： The electrocatalyst NiFeRuOx/NF, comprised of NiFeRuOx nanosheets grown on Ni foam, was synthesized using a hydrothermal process followed by thermal annealing. NiFeRuOx/NF displays high electrocatalytic activity and stability for overall alkaline seawater splitting: 98 mV@ 10 mA∙cm−2 in hydrogen evolution reaction, 318 mV@ 50 mA∙cm−2 in oxygen evolution reaction, and a cell voltage of 1.53 V@ 10 mA∙cm−2, as well as 20 h of durability. A solar-driven system containing such a bifunctional NiFeRuOx/NF has an almost 100% Faradaic efficiency. The NiFeRuOx coating around Ni foam is an anti-corrosion layer and also a critical factor for enhancement of bifunctional performances.

关键词： NiFeRuOx nanosheets     Ni foam     electrocatalysis     overall seawater splitting     solar-driven system    

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山奈酚作为新型ADAM10抑制剂治疗金黄色葡萄球菌感染 Article

王婷婷, 王建锋, 许湘竹, 蒋帆, 吕红发, 齐清辉, 张灿, 吕强华, 邓旭明

《工程（英文）》 2023年 第28卷 第9期   页码 206-221 doi: 10.1016/j.eng.2023.03.006

摘要：

宿主靶向疗法（HDT）是近年来兴起一种的治疗多重耐药金黄色葡萄球菌（S. aureus，简称“金葡菌”）感染的新策略。去整合素和金属蛋白酶10（ADAM10）作为α-毒素（Hla）不可或缺的特异性细胞受体，可通过多种机制促进金葡菌感染。ADAM10在致病机制中发挥的关键作用使其成为基于HDT治疗金葡菌感染的有力靶点。本研究首次证明ADAM10在金葡菌诱导的坏死性调亡中也发挥重要作用，这一发现拓宽了人们对ADAM10在金葡菌感染中作用的认识。通过荧光底物分解试验，我们进一步筛选出了一种强效ADAM10抑制剂——山奈酚，该化合物可抑制Hla引起的屏障破坏和细胞坏死性调亡，有效保护小鼠免受金葡菌感染。总的来说，本研究提出了一种新的宿主靶向治疗策略，并提供了有较高应用前景的候选化合物山奈酚。

关键词： 宿主靶向治疗     山奈酚     ADAM10抑制剂     金黄色葡萄球菌感染     屏障破坏     坏死性调亡    

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2014—2016年食品动物源RE-<i>cmeABCi>阳性弯曲菌流行与耐药性现状 Article

刘德俊, 刘蔚雯, 李星, 姚红, 沈张奇, 汪洋, 沈建忠

《工程（英文）》 2020年 第6卷 第1期   页码 34-39 doi: 10.1016/j.eng.2019.10.013

摘要：

弯曲菌是引起人类胃肠炎的一类重要食源性致病菌。RE-CmeABC是近年来弯曲菌中新发现的一种增强型耐药外排泵，可介导对兽医和人医临床治疗弯曲菌感染的重要药物，如氟喹诺酮类、酰胺醇类、大环内酯类及四环素类等药物的高水平耐药，而目前尚未有对该外排泵的大范围流行病学调查。因此，本研究调查了2014—2016年连续三年间我国山东、上海和广东等三个重点养殖区食品动物源RE-cmeABC阳性弯曲菌的流行与耐药性现状。结果显示，在1088株弯曲菌（931株结肠弯曲菌与157株空肠弯曲菌）中共检测出122株（11.2%）RE-cmeABC阳性菌株，包括111株（70.7%）空肠弯曲菌与11株（1.2%）结肠弯曲菌，空肠弯曲菌中RE-cmeABC的阳性率显著高于结肠弯曲菌。111株RE-cmeABC阳性空肠弯曲菌与46株RE-cmeABC阴性空肠弯曲菌相比在氟苯尼考、克林霉素和红霉素的耐药率上具有显著差异（P < 0.05），而在环丙沙星、四环素和庆大霉素的耐药率上无显著差异。然而，携带RE-cmeABC的菌株可以使环丙沙星与四环素对弯曲菌的MIC值分布趋于更高水平范围。脉冲场凝胶电泳分型（PFGE）结果显示RE-cmeABC阳性菌株在上海和广东地区以水平传播为主，在山东地区以克隆传播为主；三株分离于上海和广东的阳性菌为同一克隆型，4株分离于上海和山东的阳性菌为同一克隆型。本研究表明RE-cmeABC已在我国食品动物源弯曲菌中广泛流行，对公共卫生安全具有潜在威胁。

关键词： RE- <i> cmeABC i>     弯曲菌     环丙沙星     氟苯尼考    

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A highly efficient methodology for the preparation of

Yongxin Zhang, Shucheng Wang, Yaodong Huang

《化学科学与工程前沿（英文）》 2021年 第15卷 第3期   页码 679-686 doi: 10.1007/s11705-020-1979-9

摘要： A convenient and highly efficient method is described for the synthesis of -methoxycarbazole derivatives, including those with sterically demanding, benzannulated, or strongly electron-donating or -withdrawing substituents. Various -methoxycarbazole derivatives were directly prepared in good-to-moderate yields by the Pd (dba) CHCl /9,9-dimethyl-4,5-bis(diphenylphosphino)xanthene-catalyzed reactions of the corresponding dibromobiphenyl compounds and methoxya-mine. Based on this methodology, the first total synthesis of 3,3′-[oxybis(methylene)]bis(9-methoxy-9 -carbazole), an antimicrobial dimeric carbazole alkaloid previously isolated from the stem bark of , was achieved in 18% yield over seven steps from 1,2-dibromobenzene.

关键词： N-methoxyl carbazole     dimeric N-methoxyl carbazole     alkaloid     total synthesis     double N-arylation of methoxyamine    

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标题 作者 时间 类型 操作

Genomic regions associated with the sex-linked inhibitor of dermal melanin in Silkie chicken

Ming TIAN,Rui HAO,Suyun FANG,Yanqiang WANG,Xiaorong GU,Chungang FENG,Xiaoxiang HU,Ning LI

期刊论文

Progress in studies of sex determination mechanisms and sex control techniques in

Qian ZHOU,Songlin CHEN

期刊论文

Room temperature preparation of hydrazine-linked covalent organic frameworks coated capillaries for

期刊论文

Kinematical synthesis of an inversion of the double linked fourbar for morphing wing applications

AVILéS, I. FERNáNDEZ, M. ABASOLO

期刊论文

The secondary laticifer differentiation in rubber tree is induced by trichostatin A, an inhibitor of

Shixin ZHANG,Shaohua WU,Weimin TIAN

期刊论文

Dual cross-linked MXene/cellulose nanofiber/nickel alginate film with improved mechanical properties

期刊论文

Identification of molecular markers linked to rice bacterial blight resistance genes from

Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN

期刊论文

利用<i>cyp17a1i>缺失的伪雄鲤成功构建全雌鲤群体

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